Sentence examples for contain restriction from inspiring English sources

These primers were designed to amplify the coding region of all VEGF isoforms and to contain restriction endonuclease sites (BamHI and XhoI) for sub-cloning into pET21a vectors.

All genes encoding the signal peptides listed in Table 1 were synthesized by overlap PCR, which contain restriction sites for XbaI and EcoRI respectively.

The DNA fragment encoding the T. reesei RUT C-30 cbhII gene (GenBank accession# M16190.1) was amplified from cDNA by PCR using primers cbhF (5′-GAGATCTAAAATGATTGTCGGCATTCTCACCACG) and cbhR (5′-GACTAGTCAGGAACGATGGGTTTGCGTTTG) which contain restriction sites (underlined) for BglII and SpeI, respectively.

The primers contain restriction sites for in-frame ligation into the multi-cloning site of the vector.

A preferred expression host should not contain restriction modification enzymes that can destroy the foreign DNA, and also have low production of secreted proteases that could hydrolyse the produced proteins.

The probe did not contain restriction sites for BamH I or Hind III.

Various folders throughout the collection may also contain restrictions, which are noted in the contents list.

Furthermore, agreements with Sears's creditors may contain restrictions on assets sales.

Sometimes, Mr. Jacobs said, the bylaws contain restrictions that may bar the sponsor from casting votes for any shareholder other than a sponsor affiliate.

The military tribunal rules also contain restrictions on lawyers that will make it difficult, if not impossible, for them to mount effective defenses.

But because yeast does not contain restriction-modification systems, such transplantation poses problems not encountered in transplantation from one bacterial cell to another.