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All eight proteins contain a RING zinc-finger motif with six highly conserved cysteine residues that match the consensus sequence for the C3HC4 type (RING zinc-finger) domain group (http://www.sanger.ac.uk/Software/Pfam/).sanger.ac.uk/Software/Pfam/
Many E3 enzymes contain a RING domain.
The cytosolic domains of gp78 and Hrd1 contain a RING finger domain that harbors the E3 activity [31], [39], as well as domains that recruit the AAA ATPase protein, p97/VCP, to the ERAD complex [32] [34].
None of the α-COP homologs retrieved during our analysis were previously annotated to contain a RING finger domain.
However, these proteins only contain a RING or NHL domains, and none of them contain both domains.
The most extended proteins contain a RING finger, two B-boxes, a coiled-coil region and a B30.2 domain.
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Several IAPs also contain a RING-zinc finger domain at the carboxy terminus with autoubiquitination and degradation activity.
In particular, you may also point out homologues which are annotated to contain a RING-finger domain.
The Rabring7/BCA2/BTLs E3 ligases contain a RING-H2 domain and a C2/C2 zinc finger, and share at least two common sequence LOGOs located between the two zinc fingers (see Figure 1a) [ 19, 20].
All the members within both subgroups are required for the formation of crossovers and are similar in terms of protein structure; they contain a RING-type zinc finger domain, an internal coiled-coil domain, and a C-terminal domain that tends to be serine rich (Reynolds et al., 2013).
Both HOIL-1 and HOIP contain a RING-in-between-RING (IBR -RING (RBR) domaIBR -RINGence foRBRpart of the RBR subclass of RING-E3s, so in principle either HOIL-1 or HOIP couldomainunt for the ubiquitin ligand activity of LUBAC.
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