Sentence examples for contain a restriction from inspiring English sources

Bold sequences refer to primer tags that contain a restriction site (named in following column).

Further, MlyI and other blunt-end restriction enzymes without a cutsite in the center of a palindromic sequence (for example, Type IIS enzymes) do not have this calibration requirement as half or more of the reads will not contain a restriction motif at all.

The Senate farm bill also contains a restriction on lottery winners.

Amplification of the above mentioned genes was carried out using 5′ primers that contained a restriction Sfu I site, followed by an optimized Kozak consensus sequence (ATGG), as well as a start codon, and a 3′ primer containing an EcoR I restriction site (Table 1).

To generate Y744F, primer F3 containing a 5' restriction site for Bam HI and corresponding to nts.

The oligonucleotides contained a restriction site, (underlined, a Xba I site in the VH3-23 primer and a Sal I site in the CγB primer) for directional cloning of the PCR products.

The oligonucleotides contained a restriction site (underlined, a Xba I site in the in the VH3-consensus or VH3-23 primer and a Sal I site in the CγB primer) for directional cloning of the PCR products.

Briefly, following synthesis of double-stranded cDNA using oligo dT 18 beads, the cDNA was digested with NlaIII and ligated to a first adapter (GEX adapter 1) containing a restriction site recognized by MmeI.

For TbUNC119-TbUNC119BP double knock-down analyses, TbUNC119 cDNA (nt 1 to 468) was amplified by PCR using primers, 5'-CTCGAGCCATTCTGCATTGAATTGCG-3' and 5'-CTCGAGATGGCTGCGACGACCGAGGTC-3', which contained a restriction site for XhoI.

The forward primer contained a restriction enzyme site of SacI and the reverse primer contained a restriction enzyme site of HindIII.

The synthesis was done with a modified oligodT containing a restriction site for BsgI.