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Observations with reduced datasets suggest that ∼1000 sequences are necessary to reach comparable contact and specificity accuracy as obtained for the TCS interactions; see the insert of Fig. S3 and Fig. S5.
To directly investigate contributions of each interface contact to specificity, we determined for each mutant the k2 ratio for cleavage of the SRL RNA and the nonspecific ssNA substrate.
This count was also dissected into the number of specific contacts made, where specificity is defined as interactions between peptide side-chains and ETS residues.
A rigid-body rotation model is also consistent with the observation that specificity contacts in colicin immunity protein complexes can involve different regions of the interface.
Our findings show that, as with other interfaces, only a subset of contacts contributes to specificity.
As with other biomolecular interfaces, only a subset of contacts contributes to specificity.
Informational methods, especially those built only on pairwise contacts, necessarily lack specificity.
These experiments suggested that several residues within the cleavage loop make essential recognition contacts, while other specificity determinants are likely involved in facilitating LexA's conformational change.
Each determinant contacts a specific subset of chaperonin subunits, indicating that TRiC paralogs exhibit distinct but overlapping specificities.
This illustrates the potential for histone contacts to influence the specificity of remodeling by both targeting and altering the outcome of remodeling reactions.
A key step of adaptive immune responses is the T lymphocyte capacity to detect the presence of foreign antigens on specialized cells with high speed and specificity during contacts lasting a few minutes.
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