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Ten newly molted male phase (MP) or euhermaphrodite-phase (EP) shrimp, and 20 30 intermolt MP or intermolt EP shrimp were used for contact cue extractions.
Since none of these compounds individually elicited a response as potent as the complete newly molted EP shrimp extract (22 of 30, Table 1), we tested the hypothesis that a bouquet of CHC's functions as the contact cue.
Our study is not able to determine whether the increased resistance acts as an olfactory, as an contact cue or both.
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It has been difficult to probe the search algorithms of growth cones in response to multiple contact cues during 3-D navigation using traditional two-dimensional (2-D) substrates.
We suggest this may be because of the method of mate assessment in L. equestris, which appears to be primarily via contact cues, which may limit simultaneous comparison between options.
During embryonic neural development, axon tips ("growth cones") are guided through a dynamic three-dimensional (3-D) landscape by soluble chemotropic factors and by immobilized, growth-permissive or growth-inhibiting contact cues present in the extracellular matrix and on the surface of surrounding cells.
This further implies that the role of lipophilic cuticular hydrocarbons (CHC's) as contact cues has not exclusively evolved in insects.
Two principle classifications of sex pheromones exist: distance cues and contact cues, and examples have been identified in an ever-growing number of species.
The best studied contact pheromone systems in marine crustaceans are the harpacticoid copepods where glycoproteins were proposed as contact cues in Tigriopus japonicus [18].
Adding sound contact cues on motor performance when reaching to grasp an object facilitates and fine-tunes action performance [18], [19], [21] [24].
Utilizing the physical properties of contact cues in matrices as signal delivery mechanism could be widespread amongst species where reproduction is linked to an individual's molt.
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