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HPLC has also the limitation of large consumption of solvent volumes, which is due to the continuous flow of mobile phase and large instrumentation dimension.
In addition, it is cost-effective as consumption of solvent is reduced and is effective in removal of anti-nutritional factors, toxins and avoid degumming process [23 25].
Furthermore, green solvents are effective in consumption of solvent, reduction of downstream processing steps (reclamation of solvent) without causing any effect to other desired products.
The application of the optimized method required only 150 µL of patient plasma and a low consumption of solvent to provide rapid results.
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Continuous consumption of solvents caused both carbonate and ether-based electrolytes incompetence for the nonaqueous Li O2 batteries.
Though these analytical procedures are sensitive, it entails a disadvantage of not being simple and requiring sophisticated experimental setup, increased cost of analysis and high consumption of solvents by the mobile phase for the chromatographic techniques.
Conventional HPLC instrumentation has suffered from such challenges as long analysis time, large consumption of solvents and reagents, high cost, complicated routine maintenance, ease of use and large dimension.
Microfluidic platforms provide several advantages for liquid liquid extraction (LLE) processes over conventional methods, for example with respect to lower consumption of solvents and enhanced extraction efficiencies due to the inherent shorter diffusional distances.
With regard to cost and waste, consumption of solvents and reagents should be low.
The main advantages of using microwave irradiation at the preparation stage are reduction of extraction time, reduced consumption of solvents, and increased recovery of an isolated analyte.
In situ microarray synthesis is robust and efficient in comparison with spotted synthesis; however, the total synthesis time and the consumption of solvents and reagents are still a significant economic constraint.
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