Although we follow a hierarchical sequencing strategy, we refrain from a physical mapping-based approach because it is laborious and time consuming — sequencing would have to wait until a physical map is obtained.
However, it is too costly, inefficient, and time consuming to sequence the entire human genome.
Similarly, PCR setup and downstream sequencing processes for the MIT-NGS method are comparable to the SBT method, but less laborious and time consuming since sequencing reactions are reduced due to multiplexing and pooling (Additional file 9).
Previously, presentation of a genome on the chromosome level was done only for a limited number of yeast species, and by using expensive and time consuming Sanger sequencing [ 34].
For that reason, imprecise cross-matching or time-consuming sequence-based typing methods have generally been used to choose specific donor and recipient pairs.
The 2 sets of primers designed for the specific PCR amplification of A. ovis and A. marginale allow easy-to-detect and discriminate between the 2 pathogens, thus avoiding the time-consuming sequencing or multi-gene amplification procedures.
The observed 1B3/9B9 binding ratio was 3-fold lower for affected individuals, thus allowing identification of this particular mutation in the stalk region of human somatic ACE with high sensitivity and specificity and without labor-time and cost-consuming sequencing or restriction analysis of the PCR products [39].
We conducted an RFLP assay in order to determine 7-C carrying individuals in mtDNA D310 region by eliminating the time-consuming sequencing method.
The present molecular authentication methods, such as AS-PCR, DNA sequence analysis, and SCAR markers, often require screening new fragments or alleles to design specific primers when new adulterants are encountered; thus, these techniques require time-consuming sequencing or have limited application.
Besides being time consuming, analyzing every sequence is also not an ideal procedure (especially in this case) given the following observation.
Compared with the traditional Sanger method, which is expensive and time consuming, next-generation sequencing (NGS) technologies or massively parallel sequencing technologies (e. g. Illumina/Solexa-based RNA-Seq technology) are much simpler and more cost-effective [ 17].
