Enzyme linked immunosorbant assay is a economical, time consuming, low sensitivity method.
Blood culture is still the gold standard for definitive diagnosis of neonatal sepsis, in spite of some drawbacks of blood cultures as being time consuming, low sensitivity, and possible contamination especially with commensal CoNS that could be produced.
The method is more strategic than previous robustness analysis [52], as it focuses on parameter sets that best allow the model to match a full set of training data, avoiding time-consuming sensitivity analysis of parameter sets that cannot describe the biology of interest.
Overall, the available techniques are time consuming, have low sensitivity or require expensive capital equipment or reagents.
However, these traditional methods are time consuming, and their sensitivity for early detection is low [ 5].
The preparation of high-quality Southern blot is technically demanding, is time consuming and its sensitivity is generally low and limited to very large CNMs.
Thus, while the PCR-ELISA method was found to be both relatively demanding and time consuming, better sensitivity coupled with high specificity and speed of the assay makes the real-time RT-PCR a valuable tool for diagnosis of EAV infection.
In the μ-ECL cell, large size working electrode was still applicable, which maintained ECL detect sensitivity while consuming a small amount of sample.
Microfluidic technology seeks to improve analysis time, decreasing the consumption of sample and reagents, diminishing the risk of contamination, consuming less power, and sensitivity through automation, integrating multiplexing analysis, and especially portability to provide the possibility of point-of-care applications [38 40].
Isolation and culture of HCMV is the most specific method, but is time consuming and has low sensitivity.
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