As manual tumour delineation is time consuming, requires expertise and is prone to observer variation, automatic tools are preferable.
Identification of these fungi, based on morphological characteristics, is time-consuming, expertise-demanding, and could be inaccurate.
Serial dilution provides a simple, low-cost method for isolating individual cells from abundant cell populations but is time consuming and requires expertise [7].
The coding process is time consuming and requires expertise [ 2, 4, 6].
These indices are time consuming, demand professional expertise and training and often include application of colored plaque disclosing solutions, which are difficult to employ in regular school settings.
Obtaining such data is time consuming and requires considerable expertise.
It is also time consuming and requires technical expertise that may result in variability regarding cellular recovery.
In general, these methods require tailored programming which can be time consuming and requires specialist expertise [ 15].
This gives rise to specific and stable bonds, but the surface functionalization is generally achieved by time consuming procedures requiring qualified expertise and quite advanced technology [ 5, 6].
Although viral transduction can be effective, construction and handling of viral vectors and the processes involved in transduction are time consuming and require specialist expertise and, in cases of clinical application, safety issues can become paramount [ 1- 4].
However, current methods to quantify autophagic activity using LC3 are time consuming, labor intensive, and require expertise in accurate interpretation.
