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On two separate days, the participants consumed a test meal including 50 g iso for breakfast after overnight fasting.
The participants consumed a test drink containing 75 g glucose (Grain Process Enterprises Ltd ,Scarborough, ON) dissolved in 300 mL of water.
Subjects then consumed a test meal that contained 3.3 MJ metabolizable energy (approximately 30% of daily energy intake), with a calculated glycaemic load of 56 g and a glycaemic index of 57%.
In this small study, 27 elderly subjects consumed a test food containing Bifidobacterium longum BB536 for 5 weeks, with an influenza vaccination (2004/2005 campaign) being given at 3 weeks.
The subjects then consumed a test drink containing 75 g glucose (Glucose) (Grain Process Enterprises Ltd ,Scarborough, ON) or 75 g glucose + 24 g Oliggo-Fiber® Instant Inulin (Cargill Inc, Wayzata, MN) (Inulin) dissolved in 300 mL of water.
After fasting for at least 12 h, the participants visited the clinic in the morning and consumed a test meal prepared by the Japan Diabetes Society (450 kJal/1582 kJ; 15% protein, 35% fat, and 50% carbohydrate; 1.6 g salt 1.6 g) [ 13].
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A subset of patients (n = 118, ∼20 per group) consumed a mixed-meal test with Ensure (350 kcal; 50 g carbohydrate, 13 g protein, and 11 g fat) at baseline and after 8 weeks of treatment.
Participants consumed a standardised breakfast on test days followed by test meals: (1) control meal containing 86 g (0.5 cup) of beans, and (2) staggered meal in which 86 g (0.5 cup) of beans were consumed 15 min before the rest of the meal.
Three hours before the first and second submaximal exercise tests subjects consumed a meal, immediately after the third test subjects consumed their dinner, and after the fourth submaximal exercise test subjects were asked to sleep.
During an initial measurement the test video consumed a bandwidth of 2.78 Mbit on average.
On the evening prior to each test day, subjects consumed a standardized meal of ~1000 kcal between 18 00 19 00 h and a snack between 21 00 22 00 h.
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