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Our results show that linear DNA constructs up to several hundred base pairs were formed and could be separated from a substantial fraction of non-linear constructs.
Insulin released from the matrices increased the wet weights of the cartilaginous cell-polymer constructs (up to 3.2-fold), the amount of GAG and collagen in the constructs (up to 2.4-fold and 3.2-fold, respectively) and the GAG and collagen content per cell (1.8-fold and 2.5-fold, respectively), compared to the control.
No significant expression is detected in any of the adult muscles in constructs up to duf −3.8 kb lacZ.
None of the constructs up to those with −5.5 kb of 5' sequence are expressed in dorsal abdominal muscles or in any of the thoracic muscles.
All smaller duf enhancer constructs up to 3.8 kb show no, or comparable to background, reporter expression in both thoracic as well as abdominal muscle founder cells of the adult.
Addition of the mRNA promoter increased the activity of the constructs (up to twofold), indicating that the two regulatory elements could exhibit synergistic behavior.
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