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The ability to digitally direct and deliver cells has opened up applications in the fabrication of tissue models for studying disease pathophysiology, as complex multicellular constructs to perform drug screening and as constructs to model cancer growth.
For shRNA reporter assays, gBlocks (Integrated DNA Technologies, Inc., IDT) with shRNA binding sites were inserted into pTtNPT or pRSF91.mTagBFP2.Sensor.WPRE as described62 to generate stable 32D reporter cell lines, which were then transduced with shRNA constructs to perform the reporter assay62.
This supplemental table provides a list of oligonucleotide primers used to make DNA constructs, to perform site-directed mutagenesis, and to confirm DNA constructs by sequencing.
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A suitable cell was constructed to perform this study.
Within the PlasmonX experiment at FLAME, a spectrometer is being constructed to perform these measurements.
Within the PlasmonX experiment at LNF a spectrometer is being constructed to perform these measurements.
The talk will describe recent developments on the testbed we have constructed to perform experimental evaluation of resilience techniques for cyber-physical systems.
Many devices have been constructed to perform endoscopic suturing, but all are rather complicated, expensive, and difficult to use.
The convex lower bounding function for BP-ANN model is constructed to perform the α BB algorithm.
A volume transmission phase holographic element was designed and constructed to perform as a building integrated photovoltaic concentrator.
The finite element model of this bridge is then constructed to perform the modal analysis with original design parameters.
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