Internal ribosome entry site or 2A-peptide sequences allow such multigene expression constructs to be engineered.
Finally, we would argue for gene therapy constructs to be tested for safety in human cells.
We designed two constructs to control SEAP expression.
A Neon Transfection system (Thermo Fisher Scientific) was employed to deliver DNA constructs to generate all cell lines.
We retained the first two mifM codons in all the constructs to minimize variation in the translation initiation efficiencies.
Transposition of the expression constructs to the SBW25 attTn7 site was performed as described for the luciferase fusions.
TinyGALS is implemented in the galsC programming language, which provides constructs to systematically build concurrent tasks called actors.
With a program this size it helped to be able to use C constructs to simplify implementation.
A comparison of Rendezvous and existing primitives and language constructs to solve interprocess synchronization and communication is made.
Such warmth and deference, along with a propensity to scribble equations on window panes and wander the campus mumbling constructs to himself, makes Nash the group eccentric.
Cinekink rejects the heteronormative sexist stereotypes and instead embraces the idea that sex and gender are constructs to be played with, questioned, but most of all enjoyed.
