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RNA extracted from cell lines transiently transfected with expression constructs of the various optimized E7 genes showed a somewhat elevated expression over wild type in the two analyses conducted (Fig 5 is representative).
The expression constructs of the various SAS-6 DMs under the control of tetracycline-inducible promoter were made using the lentiviral vector pLVX-Tight-Puro vector (Clonetech) as described previously (Fong et al., 2014).
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Moreover, as described below, the recruitment of these deletion constructs to the various regions of the PCM is at least partially dependent upon the presence of endogenous full length PLP.
These changes in environment through time can be recognized in adjacent wells (via the process of correlation) and so a three-dimensional picture can be constructed of the various layers of rocks and paleoenvironments penetrated by the wells.
Borehole failure diagrams were constructed for the various degrees of stress anisotropy and a model was developed for predicting the effect of stress anisotropy on borehole failure in sandstones.
Best-fit models of evolution were constructed for the various character sets used in the partitioned analysis with MrModeltest [ 59] (Additional file 1, Table S3).
The constructs for expression of the various GST-gp78c truncation mutants were generated by a PCR-based mutagenesis approach using the pGEX-gp78c as the template and the TagMaster Site-Directed Mutagenesis kit from GM Biosciences (Frederick, MD).
Indeed, the absence of sound biological constructs or definitions of the various dimensions that make up temperament may be the primary reason why heritability values of temperament traits to date have been low.
In particular, we observed the same length for all mature mRNAs of the constructs with the various insertions.
Using fossils simply for identification purposes, Smith constructed a map of the various surface rocks outcropping throughout England, Wales, and southern Scotland.
Just as important, we could go from a 3D world back into the Web; this meant we could construct 3D models of the various resources in the Web.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com