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In our previous study (Zheng et al., 2014), we utilized constructs of the isolated fingers/palm, RH, and thumb domain to assign many of the isoleucine δ-CH3 resonances in RT.
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Based on the known structure of a similar MLL1 domain we developed homology models and designed expression constructs for the isolated CXXC domains of Dnmt1 and Tet1 accordingly.
MD constructed one of the isolated haustoria and the germinated spore cDNA libraries and provided the corresponding sequences.
No matter what type of transgenic platform is used as a bioreactor, guidelines developed by the FDA require monitoring of the animals' health, validation of the gene construct, and characterization of the isolated recombinant protein as well as the performance of the transgenic animals over several generations.
Two DIG-labeled DNA probes were constructed from the isolated RT domain of Jc3 and Jc5 using a PCR DIG Probe Synthesis Kit (Roche Diagnostics, Mannheim, Germany).
The chemical shifts for residues in common with the isolated chaperone domain are largely unchanged in S1R Δ35), and the secondary structure of the chaperone domain in S1R Δ35) is similar to that of the isolated chaperone domain construct [21].
Next the oligomeric state of the isolated HsAPC-1 RD construct was investigated.
In our study, we constructed a cAMP buffer based on the tandem cAMP-binding domains of the isolated PKA-RIβ.
The constructs were introduced into Hep3B cells and pools of stably transfected clones were isolated.
Extensive as the studies are in providing insights into incumbent-challenger dynamics (ICD), the fragmented nature of the literature and the isolated treatment of various constructs at a particular level of analysis, merit a review and analysis.
Pooled cell lines stably expressing the construct of interest were isolated by selection with hygromycin-B (500 μg/ml) over multiple passages.
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CEO of Professional Science Editing for Scientists @ prosciediting.com