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In the EGFP-Kv7.2-HA chimeric constructs, in addition to an EGFP at the cytoplasmic N-terminus, an HA epitope was inserted in the extracellular loop that connects transmembrane domains S1 and S2 of Kv7.2 subunits, as previously described61,62.

However, adolescents may also be considering other health-related constructs in addition to their physical and mental functioning when self-rating their health.

These vector constructs (in addition to an empty vector) were transfected into MDA-MB-231 and Hs578T cells to generate the KIAA1199 knockdown cells (ShA and ShB) and control (ShNC) cells respectively.

The final instrument was determined to be reliable with high alpha coefficients for each of the five distinct constructs in addition to Course Satisfaction: Website Usability (3 items; α = 0. 806), Course Usefulness (2 items; α = 0.808), Instructional Design Effectiveness (5 items; α = 0.922), Computer Proficiency (3 items; α = 0.9015) and Self-learning Attitude (3 items; α = 0.849).

Similar(56)

The electronic transfer would be achieved by introducing different histidine ionization states in selected positions of the internal core of the construct, in addition to specific mutations with charged amino acids that altogether will allow the formation of coherent networks of aromatic ring stacking, salt-bridges, and hydrogen bonds.

Two new precursor proteins were also constructed, in addition to the MINC precursor protein described above, in order to assess the versatility of the C-terminal biotinylation reaction.

Condition 6 (control group) is constructed in addition to each condition round.

The control group (condition 6) is constructed in addition to each condition round.

The Latin Square design and the control group, which is constructed in addition to all condition rounds, both serve to eliminate bias due to time.

Unexpectedly, we found that this DBD construct, in addition to forming a 1 1 high-affinity complex, can form complexes with higher than anticipated stoichiometries.

To control for individual differences in response styles, such as a personal disposition to answer negatively to questionnaires, occupational and organizational -level ecological ERI scores were constructed in addition to individual-level ERI scores.

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