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Mutagenesis is an alternative way for constructs design.
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On the contrary, the two levels of long-term institutionalized care enshrined in the Medicaid scheme are legislative constructs, designed to serve governmental cost containment policies.
Four shRNA constructs designed against HP1γ were tested in comparison to a control construct (shCTR) with at least 4 base pair mismatches to any known mouse gene.
The study presented here relates the optimization of exon exchange (ExChange) constructs designed for rescuing mutated mRNAs from very large genes such as the dystrophin gene.
We transfected primary neuronal cultures with constructs designed to block specific Gα or Gβγ subunits and assayed their effects on antirepellent signaling.
Other pioneer reports also showed efficient gene silencing in trophozoites transformed with plasmid constructs designed for episomal expression of antisense and short hairpin RNA (shRNA) [22] [24].
Using vectors of pHellsgate proved to be a straightforward method for assembly by Gateway recombination of hpRNA constructs, designed for high-throughput gene silencing in plant cells [21].
DS designed the TR3 expression constructs, designed the experiments, analyzed the data and reviewed the manuscript.
Three shRNA constructs designed against Dpy19l1 and a scrambled shRNA plasmid as a control were tested.
Multiple transgenic Drosophila strains were also generated using constructs designed to encode hApp and hApp+1 proteins (Supplemental materials).
siRNA constructs designed to match nonconserved 21 nucleotide sequences within the CCN5 mRNA were transfected into VSMC.
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