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The constructs consisted of glass and glass ceramics in the system P2O5 CaO Na2O TiO2 and they were prepared by foaming a slurry of glass particles by addition of a H2O2 solution, and subsequent sintering of the porous structures obtained.
Transient transfection of constructs consisted of 2.0 µg Kv11.1-wt or Kv11.1-mut, 1.0 µg Kv11.1-wt and 1.0 µg Kv11.1-mut or 1.0 µg Kv11.1-wt for the equivalent of a 35 mm dish.
All constructs consisted of the firefly luciferase ORF flanked by the complete UTRs of each virus.
The reporter constructs consisted of the full-length human CRE upstream of the mouse Gata2 minimal promotor driving eGFP.
The constructs consisted of a PCR insert (dsDNA between 500 and 2700 bp) and a de novo sequence (between 30 and 255 bp).
The huDR6 CRD 3-4 and huDR6 CRD 1-4 consisteds cofsisted of residues 132 213 and 42 213 of the full-length human DR6 protein, respectively, and a C-terminal 12-His tag.
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The results of a factor analysis identified six push factor constructs consisting of 19 items and four pull factor constructs consisting of 26 items.
The cultured constructs, consisting of scaffold and cells, were evaluated by gross, radiologic, and histologic examinations.
"Sterile Feral" constructs consist of a stage-specific promoter, a blocker for a critical developmental gene and a repressible element.
Subsequently, seamless and stable hybrid constructs consisting of PDSII® USP 2-0 CHCs embedded in porous collagen type I were produced.
The constructs consist of three-dimensional (3D) free-standing skeletal muscle fibers co-cultured with motor neurons.
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