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The site-specific integration tool enables not only the comparison of PREs and control constructs at the same genomic location, but also the comparison of different PREs.
In summary, site-specific integration combined with an mw reporter enables comparison of PREs and control constructs at the same genomic loci.
Differences in chromosomal location have been avoided by integrating different constructs at the same locus (16) or mitigated by averaging the behaviour of constructs at multiple integration sites (23).
Thus, to gain a quantitative understanding of differences in PRE function, and to determine the contribution of specific DNA sequences, it is essential to compare PREs, sequence variants and control constructs at the same genomic locus.
The investigation of cell forces with our strain applicator allows us to analyze mechanical properties of cell constructs at the same time as we can track visually changes in cellular morphology.
Meanwhile, a significant effect was observed for both strain frequencies and amplitudes on the expression of the four tendon-specific ECM genes of TDSCs in the cell-scaffold constructs; at the same amplitude the most obvious effect was observed in 0.5 Hz experimental group, while it was seen in 4% experimental group at the same frequency, respectively.
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The first part of this line was intended to be constructed at the same time as the City Circle Line, to avoid a multitude-higher construction cost and long interruptions of operations later.
Among other things, the two tests must measure the same construct at the same level of difficulty and with the same accuracy.
Hoover Dam was constructed at the same time, as was the Empire State Building, the tallest skyscraper in the world when it opened in 1931.
Their water absorption by capillarity was measured and compared with sixteen control cores extracted from four non-silane treated RC cross-beams constructed at the same time.
Keith Anderson, the chief executive of Scottish Power Renewables said: "East Anglia One will be the best value offshore windfarm ever constructed, at the same time as delivering industry-leading levels of UK content.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com