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First, a simple method to infer DNs using time-course gene expression data identifies DRNs over time and constructs a template PPI network with the known PPIs among all the DRNs (Hwang et al., 2009; Przytycka and Kim, 2010).
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We thus constructed a template in the same modality as the images we wanted to normalize and we spatially normalized raw FA images (with linear affine and nonlinear parameters) to this modality-specific template.
When adding lysozyme in a polymer to construct a template for protein adsorption and testing with lysozyme itself, the change of enthalpy was found to be 17.93 mJ.
Mutations of the conserved cysteine residues were obtained by inverse PCR using pET28 based constructs as a template and oligonucleotides containing specific base changes.
The NaTrxh GFP KDEL), Nβ GFP KDEL), and p11 GFP KDEL) constructs were generated by PCR using the corresponding pEG103 construct as a template.
The gene corresponding to NTD of HpDnaB construct was amplified by Polymerase Chain Reaction (PCR) using wtHpDnaB construct as a template.
The resulting constructs expressed the desired proteins with N-terminal His6-tags and TEV (tobacco etch virus) protease tag cleavage sites (extension MHHHHHHSSGVDLGTENLYFQ*SM). AKAP13 DH domain point mutant constructs were created by PCR using the His6-tag DH PH domain expression construct as a template.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com