Following vector construction, transformation and zeocin amplification, the best Pichia producers were identified in a screening procedure using Western blot and a Luminex xMAP™ based high-throughput method.
In this paper we describe an approach for the automated derivation of correct-by-construction transformation implementations from high-level specifications.
The series ends with Gitais 2007 film Disengagement, which more directly engages with the overall theme of our screenings regarding the plan, construction, transformation, and destruction of ideological settlements.
Another new acquisition is Representing medieval genders and sexualities in Europe: construction, transformation, and subversion, 600-1530, likewise a collection of essays, this time inspired in part by a 2005 meeting of the Gender and Medieval Studies Conference.
XN, WT and WH conducted the plasmid construction, transformation, RT-PCR and real-time PCR analyses, phenotypic assay and GC-MS analysis.
Standard cloning and yeast techniques were used for construction, transformation, and integration of plasmids and construction of yeast strains (Sambrook et al., 1989; Longtine et al., 1998; Guthrie and Fink, 2002).
We applied yeast based recombination mediated vector construction, transformation by electroporation and subsequently removal of the NHEJ-deficient background by crossing with a sexually competent T. reesei strain derived from QM9414.
VV helped with the plasmid constructions, Lotus transformation and time-course analyses.
Construction and transformation of Arabidopsis with the ETR1 constructs tETR1, ETR1 1-349), and ETR1(G2) was also previously described [12], [42].
SG carried out most of the experiments: vector construction, genetic transformation, selection and analysis of plants; WO coordinated some experiments (vector construction, analysis of expression) and took part in discussion of the project; ANO have made substantial contributions to conception and design, analysis and interpretation and wrote the manuscript.
