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Primer sequences were used for plasmid constructions in this study.
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Sensitivity analysis revealed the daily maximum precipitation to be key factor for the robustness of the results for three RWH systems construction in this study.
andrzejowskianus were genotyped for map construction in this study.
Furthermore, it has to be stressed that very strict criteria were used to carry out map construction in this study.
Because 8 individuals were used in library construction in this study and around 3000 EST sequences were available, it was possible to identify high quality SNPs.
Another limitation is that that many polymorphic GBS SNP markers not classified as SDA were not used for the haplotype map construction in this study.
Furthermore, 2,442 (64.8%) SNP markers and 44 (49.4%) polymorphic PCR markers that excessively missed or distorted were excluded for map construction in this study, while more than 65.4% markers were discarded for their unexpected segregation patterns that reported by Zhang et al. [ 29].
All further plasmid constructions used in this study were based on these two initial plasmids.
Standard molecular biology techniques were used to generate the different constructions used in this study.
All constructions used in this study were sequenced and confirmed to be correct.
Therefore, the network construction adopted in this study for temporal dynamics is more similar to the construction adopted in Sivakumar and Woldemeskel (2014) and Halverson and Fleming (2015) for spatial dynamics than to the one adopted in Tang et al. (2010), Braga et al. (2016), and Serinaldi and Kilsby (2016) for temporal dynamics.
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