Sentence examples for construction was done using from inspiring English sources

The plasmid construction was done using conventional techniques, i.e. PCR, endonuclease restriction and ligation (Sambrook and Russell 2001).

Most of the construction was done using hand tools. of earth was moved, of rock was blasted and 2.3 km of tunnel were built. of ballast stone were used, most of it from the gravel pits at Bevergrenda, Guribråten and Stevningsmogen, Tjuvhaugen and Skarpsmoen.

Strain construction was done using routine protocols.

Phylogenetic tree construction was done using Neighbor-Joining, bootstrapped tree inference and using the Dayhoff Matrix (Amino Acid) as a model, pairwise deletion for gaps, homogenous pattern among lineages, uniform rates among sites and all substitutions included.

Alignment for tree construction was done using ClustalW with the Gonnet scoring matrix.

cDNA synthesis and library construction was done using the Creator SMART cDNA Library Construction Kit (Clontech) according to the manufacturer instructions.

Filter bank construction is done using DB-4 Daubechies 9/7 wavelets.

The statistical analyses for the harvest rate curve construction were done using SPSS 14.0 (SPSS Inc, Chicago, IL, USA) statistical software.

The construction of cDNA libraries was done using the SMART cDNA library construction kit (BD Clontech, USA) according to the protocol provided by manufacturer, with some modifications.

The measurements of AuNPs and construction of a histogram was done using OLYMPUS software MEASURE IT tool.

Construction of phylogenetic trees was done using the Neighbor joining method.