Sentence examples for construction vectors from inspiring English sources

The library construction vectors were 8.1 kb CopyControl pCC1BAC HindIII Cloning-Ready Vectors and 8.1 kb CopyControl pCC1BAC BamHI Cloning-Ready Vectors (Epicentre, Madison, WI, USA).

Information about plasmid construction, vector, inserts and primer sequences is available in Supplementary Methods S1.

Pten PCR products were inserted into the pGC-LV vector through restriction enzyme Age I linearization, and then the construction vector which carried the Pten gene fragment was transformed into DH5α competent cells.

The promoter PN25 sequence was amplified from the plasmid pGro11 (Nishihara et al., 1998) using primer P1 and P2 (Supplementary file 1B) and subcloned into the EcoRI/ SacI sites of construction vector pMOD5 from Epicentre to create pRF1.

This genome structure and replication strategy is amenable to the construction of expression vectors.

IN assisted in construction of expression vectors.

Plasmids pNitAph and pNitX are universal starting plasmids for the construction of expression vectors for Volvox.

After construction, all expression vectors were verified by DNA sequencing (GATC Biotech, Konstanz, Germany).

The construction of expression vectors is given in detail in Additional file 2: Table S1.

(cell experiments), Y.M. (vector construction), H.O. (vector construction), N.K. (vector construction), Y.S. (lectin microarray), A.U. (supervision of cell experiments), J.H. (lectin microarray), K.H. (computational analysis and manuscript preparation), and M.A. (project leader and coordination).

A diagram depicting the vector construction of vectors used in this study is illustrated in Figure 1C.