Sentence examples for construction vector from inspiring English sources

Information about plasmid construction, vector, inserts and primer sequences is available in Supplementary Methods S1.

In brief, expression cassettes containing the CAT and GFPuv genes under regulation of the R. rickettsii isolate Hlp#2 ompB gene promoter and ompA gene transcriptional terminator were assembled and inserted into the pMOD-2 transposon construction vector (Epicentre, Madison, WI) to obtain the pMODompBCAT\GFPuv700 (pMOD700) rickettsial transformation vector.

Pten PCR products were inserted into the pGC-LV vector through restriction enzyme Age I linearization, and then the construction vector which carried the Pten gene fragment was transformed into DH5α competent cells.

The promoter PN25 sequence was amplified from the plasmid pGro11 (Nishihara et al., 1998) using primer P1 and P2 (Supplementary file 1B) and subcloned into the EcoRI/ SacI sites of construction vector pMOD5 from Epicentre to create pRF1.

The library construction vectors were 8.1 kb CopyControl pCC1BAC HindIII Cloning-Ready Vectors and 8.1 kb CopyControl pCC1BAC BamHI Cloning-Ready Vectors (Epicentre, Madison, WI, USA).

The possibility of proteolytic release was designed at the stage of expression vector construction.

(XLSX 9 kb) Additional file 4: Primers used in transient expression vector construction.

SL has participated in expression vector constructions.

This genome structure and replication strategy is amenable to the construction of expression vectors.

IN assisted in construction of expression vectors.

Plasmids pNitAph and pNitX are universal starting plasmids for the construction of expression vectors for Volvox.