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Following vector construction, transformation and zeocin amplification, the best Pichia producers were identified in a screening procedure using Western blot and a Luminex xMAP™ based high-throughput method.
XN, WT and WH conducted the plasmid construction, transformation, RT-PCR and real-time PCR analyses, phenotypic assay and GC-MS analysis.
We applied yeast based recombination mediated vector construction, transformation by electroporation and subsequently removal of the NHEJ-deficient background by crossing with a sexually competent T. reesei strain derived from QM9414.
Standard cloning and yeast techniques were used for construction, transformation, and integration of plasmids and construction of yeast strains (Sambrook et al., 1989; Longtine et al., 1998; Guthrie and Fink, 2002).
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In this paper we describe an approach for the automated derivation of correct-by-construction transformation implementations from high-level specifications.
BL carried out all the constructions, transformation and data collection.
Construction and transformation of Arabidopsis with the ETR1 constructs tETR1, ETR1 1-349), and ETR1(G2) was also previously described [12], [42].
Forever young, forever good-looking, forever sexy - beauty is still a construction, a transformation, a masquerade.
HY and WC performed the plasmid construction, plant transformation and mutation detection.
Primarily, this was mathematics including linear measurement, geometry (perspective, shape construction and transformation, direction), and calculations.
Finally, we discuss computational support for the proposed approach in terms of conceptual model construction, automatic transformation and temporal querying.
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