Sentence examples for construction template from inspiring English sources
The new systems require just 15 minutes of manual setup time per sequencing run and less than 45 minutes of hands-on work from DNA to data when using the Ion Chef System for automated Ion AmpliSeq library construction, template preparation, and chip loading.
We acknowledge the dedicated efforts of the quality systems, library construction, template, and sequencing teams at the Joint Technology Center of the J. Craig Venter Institute.
Detailed methods describing DNA isolation, library construction, template preparation, automated cycle sequencing and metagenomic assembly can be found in Rusch et al. (2007) [41] and Venter et al. (2004) [40].
MK, AMM, HK, KH and RR contributed to library construction, template preparation, sequence determination and assembly.
MK, SK and RR contributed to RNA isolation, library construction, template preparation, sequence determination and assembly.
Library construction, template preparation, sequencing, assembly, and data analyses were performed as described previously [ 19, 20].
Following the first stage of sequencing procedure, library construction, the template was prepared through emulsion PCR automated system and then run on the PGM to accomplish the sequencing process (no. of reads was 1,624,672; no. of generated contigs was 168 and achieved overall depth of coverage was 59×).
The Illumina MiSeq (for library validation) and HiSeq 2000 sequencing systems (Illumina, CA, U.S). were used for sequencing, and the library preparation kits were purchased from New England Biolabs (MA, U.S .. Sequencing library construction and template preparation were performed according to the NEB library preparation protocols.
In the long term, he said, it was essential that the plants under construction become templates for building more.
We will describe the details of the proposed scheme, including the feature extraction, the construction of templates, and the classification of highlights in the following sections.
