Table 2 Promoters used in plasmids constructions Promoter Name Accession numbers (GenBank) S288C JPU CYC1 CYtochrome C KY704470 KY704471 Translationation Elongation Factor KY704476 KY704477 PhosphoGlucoIsomeraseerase KY704472 KY704473-PhosphoGlycerateycerate KY704474Y704474 KY704475 ADH1 Alcohol DeHydrogenase KY704468 KY704468.
The reported method allows for rapid construction of promoter, 5′UTR, terminator and trans-activating RNA libraries.
For OsSHR1 GUS vector construction, the promoter of OsSHR1 was amplified by PCR and digested at the site of Hin d III and Kpn I, then inserted into pBI101.3.
Primers used for cDNA synthesis, RT-PCR analyses and construction of promoter::GUS reporters.
With respect to the experimental techniques, the construction of promoter libraries seems promising [ 5, 9- 16].
The construction of promoter:: uidA fusions and their transformation into plants were conducted as previously described.
Technical details including primer design, saturation mutagenesis, construction of promoter libraries, phenotypic analysis, data analysis, and interpretation are discussed.
Four binary transformation vectors (pBI-GFP[S65T] [ 98], pBI-1GFPB, pBI-n1GFP, and pBI-n2GFP) were used in the construction of promoter fusions with reporter genes GFP, n1GFP, and n2GFP (Additional file 10); these vectors were generated as follows.
EC carried out the construction of promoter-reporter plasmids, plant transformation and drafted the manuscript.
QS and LZ did the construction of promoter-reporter plasmids, plant transformation, transgenic Arabidopsis analysis and Agrobacterium-mediated GUS transient assay in tobacco leaves.
