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ÖÖ contributed to construction of the microarray.
JS sequenced the SSH clones and assisted in the construction of the microarray.
ND led the experimental design and framework development for the construction of the microarray.
AB performed the M-CGH hybridizations of the fifteen serovars strains and assisted with the construction of the microarray.
WAF managed the overall bioinformatics, analysed the microarray data from the 15 serovars, and contributed to the design and construction of the microarray.
The construction of the microarray probes has been based mostly on tobacco EST, cDNA and mRNA sequences, and it was necessary to verify the gene names provided by Agilent.
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TH contributed to the construction of the microarrays, the microarray hybridizations and analysis.
VRI provided the construction of the microarrays and tools for the statistical analysis.
Independent colonies from both libraries were selected for the construction of the microarrays at the Australian Genome Research Facility (http://www.agrf.org.au/).au/
Details on the construction of this microarray (AppChip1) are available on the Institute for Biological Sciences website [ 77]. cDNA synthesis and microarray hybridizations were performed as described [ 78].
After construction of the tissue microarray, every 30th tissue microarray slide was evaluated by a pathologist (H-J G).
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