Sentence examples for construction of the expression from inspiring English sources

E. coli infected with the viral library were grown on NZY agar plates in order to express the library, as described in Construction of the expression library section.

Fig. 2 Schematic diagram showing the strategy for construction of the expression vector pPIC9-cbhII2AeglII.

Therefore, screening of the signal peptide must be considered during the construction of the expression vector for the host.

For construction of the expression vectors for the E. coli strains (DH5α; BL21-Gold (DE3)), and the plasmids pET28a or pET22b (Novagen; Darmstadt, Germany) were used.

Proof From the construction of the expression S 4 ( u h, φ ) it follows that u ˆ h = u h on Π h, and u ˆ h = φ j on γ j h, j = 1, 2, 3, 4. The continuity of u ˆ h on Π follows from the continuity S 4 ( u h, φ ) on each closed triangle Type A and Type B, and from the equality u ˆ h = u h on Π h.

As it has previously been shown that the NA stalk can have an impact on NA catalytic activity [24], [25], [26], [27], these amino acids were included in the construction of the expression system, especially as they represent a predicted glycosylation site for various NAs.

The construction of the GAD-expression plasmid pCH-gadB, C. glutamicum GAD (strain ATCC 13032 harboring pCH-gadB), and C. glutamicum W (strain ATCC13032 harboring pCH) was reported (Takahashi et al. [2012]).

Here I do not refer to the construction of the co-expression network or the estimation of between-gene correlations.

The detailed methods for the construction of the γ2-expression vectors and the protein purification are described in Document S1.

The grapevine microarray data set for the construction of the co-expression network was obtained from Gene Expression Omnibus (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi acc=GPL1320) (platform accession number GPL1320).

GH participated in the construction and expression of the antibodies, worked on the in vivo local anaphylaxis assay, and participated in the study design.