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In a statement on Friday, SPR said it had found that construction of the array could be impacted by ground conditions at the site, particularly the presence of hard rock, coupled with "challenging wave conditions".
The total genetic coverage of each of the seven different strains used for construction of the array was calculated [16], [24] (Table 3).
The electrodes are 92 µm in diameter, reside in a 16×64 grid, and can be set to a specified voltage via connection to a PC with appropriate software, allowing different patterns of on and off electrodes to be used at each step in the construction of the array.
Following the construction of the array block, 5 μm sections were cut with a microtome.
20 After the construction of the array block, all the tissue blocks were cut with a microtome to 4 μm and affixed to the slide.
After construction of the array block, multiple 5-μm sections were cut with a microtome and placed on polylysine-coated slides.
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Construction of the arrays was performed by Roche NimbleGen Inc. (see reference [ 35] for additional details).
The rat genome has been sequenced since the construction of the arrays therefore bioinformatics analysis was carried out to validate the results from the microarray analyses.
The construction of the arrays (385 000 oligonucleotides), the labeling of DNAs, the microarray hybridization, and scanning were performed by the Roche NimbleGen, Inc. Core Facility (WI, USA) as described previously (11, 39).
The design and construction details of the array are discussed in addition to the measurement considerations required for appropriately characterizing an array when using ULOI amplifiers.
On this basis, conclusions were drawn in terms of the participation of various construction parts of the array of piezoelectric transducers in the process of creating the mechanical crosstalk.
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