The results showed that correct construction of recombinant pEGFP-β-actin has been shown by restriction enzyme digestion.
Combined with the construction of recombinant rodent malaria parasites14,15,16 that express the GFP at the pre-erythrocytic stages, these developments now permit in vivo analysis of Plasmodium behavior in rodent systems.
We have designed new vectors for the construction of recombinant adenoviruses containing the early region 1A (E1A) gene under the transcriptional control of heterologous promoters.
In this article, we discuss the safety of microorganisms used as hosts for enzyme-encoding genes, the construction of recombinant production strains, and methods of improving enzyme properties.
Several ligation-independent cloning methods have been developed that offer advantages for construction of recombinant plasmids at high efficiency while minimizing cloning artifacts.
Here we report the design of a method enabling the construction of recombinant proviral HIV-2 DNAs in a moderate copy number plasmid that allows the analysis of env gene structure and functionality.
Metabolic engineering and construction of recombinant Escherichia coli strains carrying feruloyl-CoA synthetase and enoyl-CoA hydratase genes for the bioconversion of ferulic acid to vanillin offers an alternative way to produce vanillin.
To facilitate the rapid, efficient, and uniform construction of recombinant DNA molecules, a novel cloning strategy, the univector plasmid-fusion system (UPS), has been developed based on the Cre loxP site-specific recombination system of bacteriophage P1.
To explore the function of SelK in the process of immune response, several short-hairpin RNAs (shRNA) were designed for the construction of recombinant plasmids to down-regulate the expression of SelK gene in vitro.
Fig. 2 Construction of recombinant vector for dhaD expression.
Fig. 1 Construction of recombinant strains and Gox expression.
