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Recently, high-throughput methods (e.g. yeast-two-hybrid [ 12, 13]) for detecting pairwise protein-protein interactions (PPIs) en masse have enabled the construction of PPI networks on a genomic scale.
In recent years, there have been many studies focusing on the discovery of schizophrenic candidate genes and the construction of PPI networks and related pathways for the hope of a better understanding of schizophrenia.
However, high coverage and reliable protein interaction maps for C. albicans and zebrafish would still benefit the construction of PPI networks and the investigation of essential functional modules in C. albicans infection in the future.
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Step three: Construction of PPIs network and extraction the giant component from the extended network.
LH made substantial contributions to construction of PPIs, analysis of PPIs, was involved in drafting the manuscript or revising it critically for important intellectual content, and gave final approval of the version to be published.
LH made substantial contributions to conception and design, construction of PPIs, analysis of PPIs, was involved in drafting the manuscript or revising it critically for important intellectual content, and gave final approval of the version to be published.
RJ made substantial contributions to conception and design, construction of PPIs, analysis of PPIs, was involved in drafting the manuscript or revising it critically for important intellectual content, and gave final approval of the version to be published.
The construction of weighted PPI network and the selection of seed in MCSE improve its efficiency.
YY, ZS, SH, and LG contributed to the construction of the PPI databases and networks.
Using TBLASTN, similarities between G. sinensis unigenes and genes in a PPI network consisting of six String database genomes (Arabidopsis thaliana, Arabidopsis lyrata, Oryza sativa subsp. japonica, Brachypodium distachyon, Populus trichocarpa, and Sorghum bicolor) facilitated the construction of a PPI network of G. sinensis.
Therefore, the construction of a PPI-focused chemical library is an inevitable necessity for future drug discovery.
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