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Total RNA from the different tissue was used for the construction of indexed mRNA libraries using KAPA Stranded mRNA-Seq Kit Illumina platform (KAPA Biosystems: KR0960).
In order to investigate the MEP, carotenoid, and bixin pathways genes, total RNA from young leaves and two different developmental stages of seeds from B. orellana were used for the construction of indexed mRNA libraries, sequenced on the Illumina HiSeq 2500 platform and assembled de novo using Velvet, CLC Genomics Workbench and CAP3 software.
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Following the construction of the coding framework, themes were indexed and segments of the transcripts sorted into the relevant categories using NVivo 9 software.
One is the merits of indexed investing.
Explaining the mechanics of indexed options can also be complex.
Visual readings, CSBR and indexed CSBR measurements had significantly lower specificity than the 92% specificity of Indexed CAQ (Table 4).
An infinite number of indexed equations are generated using an orthogonalization procedure.
So if you are now, say, 65, are still working, and you started work at 16, you have 45 years of indexed earnings and four years of non-indexed earnings.
Library construction was performed as described previously, using 50 200 ng of DNA sheared by sonication to B100 400 bp before end-repair, dA addition and ligation of indexed, Illumina™ sequencing adaptors (7, 8).
EZ-Text software facilitated tabulation of frequencies of indexed transcripts.
Let r be the number of indexed k-mers.
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