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Furthermore, specific pore structures and tunable morphology allow the construction of affinity probes for various macromolecules [40].
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The GESR-LR makes full use of the supervised learning information in the construction of the affinity matrix, and the affinity construction is combined with graph embedding in a single step to guarantee the global optimal solution.
And the supervised learning information is also used in the framework to guide the construction of the affinity graph.
In this paper, the construction of the affinity and graph embedding are combined to ensure the overall optimal solution.
For the construction of two affinity maturation antibody gene libraries, the DNA encoding the anti-MUC1 binder IIB6 was amplified four times by error-prone PCR to introduce random mutations.
Although not explored here, it is highly conceivable that type 7 offers an attractive alternative to current type 3 display for construction of and affinity selection from large peptide libraries [24].
GST fusion proteins were used for affinity column construction for affinity purification of antibodies.
In the construction of the second affinity maturation library, scFv(A3) was used as template.
We have devised a method, Semantic Similarity-Integrated approach for Modularization (SSIM) that integrates various gene-gene pairwise similarity values, including information obtained from gene expression, protein-protein interactions and GO annotations, in the construction of modules using affinity propagation clustering.
Expressed OR genes were fabricated from scratch using PCR-based gene synthesis (polymerase construction and amplification, aka PCA), which facilitated codon optimization for high level expression and attachment of affinity tags for detection and purification.
The effect of these DNA shape features on DNase I cleavage is due to the recognition of electrostatic potential in the DNA minor groove through arginine residues [ 68]. Figure 2A illustrates the construction of the 'shape-to-affinity model' used by Lazarovici et al. [ 63].
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