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An example we observed: there is one strain by which the library construction is biased towards to some regions.
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This is consistent with the knowledge that current cDNA library construction protocols are biased against 5' UTRs.
The clinical use of array-based diagnostics is relatively late in coming; this is partially due to the demand of a substantial number of patient samples to be used for training, since estimates of a predictor's error rate during model construction are more prone to be biased for small datasets[17].
Second, estimates should not be based solely on species tree construction methods that appear to be biased toward certain types of topologies (e.g., MDC).
On the other hand, the presence in the venom of toxins not represented in the transcriptome clearly indicates that construction of the cDNA library was biased, i.e. due to the necessary fractionating steps to avoid interfering substances like short, partial length 3'-end cDNAs and adapter sequences [ 34].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com