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This report describes the construction, expression, and in vitro function of DT5F11.
The construction, expression and characterization of the cel9E xyn10A bifunctional gene fusion obtained by site-directed mutagenesis are also reported.
Here we describe details of the construction, expression and evaluation of this scFv, which was derived from a high-affinity hybridoma (Qu) specific for the C1q globular heads.
This review discusses the principle underlying M1GS-mediated gene inactivation and methodologies involved in effective M1GS construction, expression in vivo and emerging prospects of this technology for gene therapy.
The procedure for construction, expression and screening of random viral peptide libraries using yeast surface display is shown in Fig. S1 (see also Materials and Methods for details).
Plasmid construction, expression and purification of recombinant Tpt1, preparation of sepharose-linked Tpt1 and screen for new interaction partners with ES cell extracts was performed according to previously published material [24].
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That Augie is Jewish and speaks an English in which Yiddish inflections, constructions and expressions are heard is part of what makes him, as he describes himself at the novel's end, "a Columbus of those near-at-hand".
The orderliness, the elegant patterns, the neat categories and the mathematical expressions are constructions of scientists.
For details of strain construction, gene-expression analysis, and imaging methods, see the Supplemental Experimental Procedures.
Data from construction of expression-clones and protein localisation was processed as described [ 44].
Plasmid constructions, protein expression and purification, fluorescent analysis, and additional ESI-MS spectra.
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