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One of the investigators refers to that as the "Luke, I am your father" moment: it became clear that there was only one construction cell and that Quirino was its boss.
JD carried out plasmid DNA construction, cell culture and molecular analysis.
Strain construction, cell growth, yeast transformation, spotting assay followed standard protocols (Guthrie and Fink, 2002).
Those experiments for plasmid construction, cell transfection, reporter gene assays and site-directed reporter gene mutation were performed as described previously [ 30].
Those experiments for plasmid construction, cell transfection, reporter gene assays and site-directed reporter gene mutation were performed as described in a previous study [ 22].
While the M1 macrophages promote inflammation, extracellular matrix destruction, and apoptosis, the M2 macrophages promote extracellular matrix construction, cell proliferation, and angiogenesis.
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This seemed to confirm the investigators' assumption that the construction cells were unrelated.
The agents believed that the various tunnels were being built by construction cells that were loosely affiliated with Sinaloa but unrelated to each other.
Thus, in the HS-Nintra construction, cells have enough time to elaborate their response when the N-pathway was directly activated during the S- or the G2-phases.
Amongst those, 4 glucan-binding proteins (Gbps) are considered to be important factors due to their glucan-binding properties, of which GbpB has been shown to participate in cell-wall construction and cell separation.
In this paper, we describe the design and fabrication of a dielectrophoresis (DEP -based cell-positioning anDEP -basedture device for the construction of cell networks.
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