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RNA-seq library construction and sequencing were performed by Filgen (Nagoya, Japan).
The PCR products of these samples were divided into four pools for library construction and sequencing.
Library construction and sequencing were performed by The Ramaciotti Centre (UNSW, Kensington, NSW) with TruSeq chemistry on a HiSeq2000 (Illumina).
It lets you discover ways of construction and sequencing and optimizing shapes for cost.
(C) cDNA samples from two single cells were subjected to two independent library construction and sequencing in two batches.
Library construction and sequencing were performed on a BGISEQ-500 by Beijing Genomic Institution (www.genomics.org.cn, BGI, Shenzhen, China).
A description of the library construction and sequencing performed at the JGI can be found at http://www.jgi.doe.gov.gov
Preparation of genomic DNA, library construction and sequencing was as previously described for B. hyodysenteriae [15].
Our initial library construction and sequencing was based on a rather small number of clones from non-normalized libraries [19].
All general aspects of library construction and sequencing performed at the JGI are described at http://www.jgi.doe.gov/.gov/
One sample of each triplicate series of samples (repetition number one) was used for SSU rRNA library construction and sequencing.
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