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EK performed library construction and sample normalization.
Using the results from these experiments, we explored data handling approaches for standard curve construction and sample quantification, with particular attention to the treatment of valid negative observations.
Removal of the primer-dimers from the SOLiD library construction and sample indexing step was necessary to reduce the carryover of these products into the library.
Variation is due to a mix of factors, including non-standardized criteria to determine cutoff, potential biases from library construction and sample size and increasingly better representation of dinoflagellate genes in the databases.
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Laboratory testing using blank solutions shows minimal sources of contamination from materials used in the construction and sampling procedure (i.e. titanium, MilliQ water and aqua regia).
Most likely, differences in library construction and sampling rather than intrinsic differences between the species explain this discrepancy.
The size of the full sample (N = 3,200) was sufficiently large for splitting this sample into a construction and validation sample to perform an Exhaustive CHAID analysis in which the total group of juveniles was divided into a number of smaller subgroups.
Second, we assessed the discriminatory accuracy of the Y-ACNAT-NO by calculating AUC values in both the construction and validation sample, and by examining the sensitivity, specificity, and diagnostic odds ratio at various cutoff scores in the validation sample.
No significant differences were found between the construction and validation sample in the prevalence of (specific types of) care needs.
bChi-square tests with Yates' correction were conducted to determine differences between the construction and validation sample.
The first two stages are integral to the process of psychometric development through the application of draft tools to a construction and validation sample (see below).
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construction and demolition
construction and commissioning
construction and landscaping
construction and oil
construction and acquisition
construction and consumption
construction and retailing
construction and furnishing
construction and decoration
construction and opening
construction and planning
construction and use
construction and architecture
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