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There has been considerable tension between the I.O.C. and Greek authorities concerning the disappointing pace of construction and preparation.
Construction and preparation are just re-starting at the Linen Mill Studios.
Construction and preparation of the full-length cDNA library has been described previously [20].
Detailed information on the construction and preparation of the microarray has been previously described [12].
Construction and preparation of the BCG.HIVA222 stock was described previously [26].
C Cl conducted the tissue microarray construction and preparation of data for analysis.
Similar(49)
But Mr. Broad also noted that he and Mr. Govan have talked almost daily, and sometimes several times a day, during the final phase of construction and preparations for the opening.
The construction, design and preparation of pFLAG CMV4 mammalian expression vectors (Sigma-Aldrich) expressing M. tuberculosis Rv1818c CMV1818cc) and Rv3812 CMV38122) has been described previously [35].
Although the organization adopted recommended guidelines on ship recycling in 2003, the IMO's senior technical body agreed in 2005 to develop legally binding regulations for the design, construction, operation, and preparation of ships to enable safer and more environmentally sound recycling, along with rules for enforcing the instrument.
The Illumina MiSeq (for library validation) and HiSeq 2000 sequencing systems (Illumina, CA, U.S). were used for sequencing, and the library preparation kits were purchased from New England Biolabs (MA, U.S .. Sequencing library construction and template preparation were performed according to the NEB library preparation protocols.
E. coli strain DH5α was used for plasmid DNA constructions and preparations using standard techniques as described [ 42] and were grown in LB broth supplemented with apramycin (50 μg/mL).
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