Sentence examples for construction and mutation from inspiring English sources
To deeper interpret the function of collected data, a series of functional analyses were performed, which included enrichment analysis, mutation annotation, gene disease network construction and mutation spectrum.
By adapting a workflow of TALEN construction protocols and mutation screening approaches for use in Platynereis, we engineered frameshift mutations in three endogenous Platynereis genes.
A standard error-prone PCR protocol was employed for library construction, and the mutation rate was controlled by adjusting the concentration of manganese ions.
PV therefore proves an excellent candidate for the rigorous construction of a computational model of virus replication to predict population structure and mutation distribution.
HY and WC performed the plasmid construction, plant transformation and mutation detection.
E. coli DH5α (TAKARA) was used for plasmids construction, phenotype evolution, and mutation rates evaluation.
DNA sequence analysis was performed to verify the construction and the absence of mutations.
As there are no published reports of construction and characterization of null mutations in these highly transcribed σB-dependent genes, our data clearly suggest that σB and the σB regulon make additional important contributions to L. monocytogenes physiology that remain to be characterized.
The utility of this approach in the molecular analysis of recombination intermediates and in the rapid construction of mutation spectra is demonstrated.
Specifically, we engineered i) central carbon metabolism, ii) 3HP synthesis, and (iii) 3HP tolerance through design, construction and testing of ~ 162,000 mutations across 115 genes spanning global regulators, transcription factors, and enzymes involved in 3HP synthesis and tolerance.
Her major research project entailed the construction and characterization of various PREX2 mutations associated with human cancer and their consequences for tumorigenesis and development in transgenic mouse model.
