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The contigs and annotated gene sets of T. urartu and Ae. tauschii have been useful for constructing the draft genome of common wheat [ 24].
There is also a possibility that some of the FLNC reads in G4 and G5 may come from divergent variety-specific genes since the elite common wheat variety used here (i.e., Xiaoyan 81) may differ from the land race CS used for constructing the draft genome sequence.
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We previously constructed the draft genome of Ae. tauschii, and found that it was particularly rich in genes related to adaptation16.
Then, contigs were aligned with the reference genome to construct the draft chloroplast genome of each species in Geneious 9.0.5 software (http://www.geneious.com).
Having constructed the draft PMOS measure, it will be important to explore its acceptability and face validity.
Using the alignment order of bovine Y chromosome, we constructed the draft assembly of wild goat chromosome Y (see the Additional file 1: Supplementary Methods 3.1 for further details).
An in-house program was developed using BLASTX algorithms by comparing with five green algal lineages and one high plant species to construct the draft transcriptomic database of D. tertiolecta for RNA-Seq analysis and further target gene manipulation.
After constructing the first draft of the instrument, cognitive interviewing [ 28] with read-aloud/think- aloud methods of the content was carried out with three women who met the inclusion criteria for the study.
SI, ND & JM lead the work-package on model development; MV-D & YE co-ordinated the IMPACT project; VRP, RS, EM, JB, LR, DH & LM contributed to data gathering and analysis at national sites; all authors contributed to constructing the models and drafting this report.
JU constructed the first draft and conducted the literature search.
JE constructed the first draft of the manuscript with subsequent inputs and revisions from OB and PK.
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