For analysis of germ cells, we confirmed our phase contrast data by using a constructed stock that marked the mitotic germ cells with GFP (Sano et al. 2002).
Similarly, F1 males from the cross between the UAS-RNAi construct stocks and the Dcr-GAL4 driver stock (25708) were used as second tester males.
To better visualize the location of the mature spermatids in the testes and storage structures we constructed stocks that expressed a Don Juan GFP fusion protein in a triple knockout background.
Hadavandi et al. (2010) presented an integrated approach based on genetic fuzzy systems (GFS) and artificial neural networks for constructing a stock price prediction expert system.
But simply buying a respectable Euro fund won't necessarily give investors a balanced exposure, says Dimitris Melas, executive director of MSCI Barra, a firm that constructs stock indexes.
Based on the records of order matching among investors, we can construct a stock trading network for each trading day, in which the investors are mapped into nodes and each transaction is translated as a direct edge from the seller to the buyer with the trade size as its weight.
Using the cold-shock technique, we constructed a stock of C(2 EN bearing the X-linked mutant w and the third chromosome balancer, TM6B, Tb,Hu,e.
Relative transcript levels were determined from the relative standard curve constructed from stock cDNA dilutions, and divided by the target quantity of the calibrator following the manufacturer's instructions.
Constructed rescue stocks were discarded after data were collected (due to instability) and were constructed anew for each experiment.
Constructing these rescue stocks meant introducing another balancer into the genotype to ensure the transgene would be present, which enhanced the balancer breakdown issue we had noted earlier.
