The target PCR products (pET-28a-Aoxyn11A G21X) were digested by DpnI, and transformed into E. coli BL21, thereby constructing a site-saturation mutagenesis library (E. coli/Aoxyn11A G21X).
The Thai military is reportedly financing and helping to construct a site in Laos for Hmong refugees, but "the Hmong will almost certainly resist" forcible repatriation, he said, and "there will be Hmong suicides to avoid being forced back to Laos.
In this respect, the Institute for Technological Research IPTT) has an already ongoing plan to construct a site in the state of São Paulo to promote the development of thermochemical technologies [57].
Instead of transporting all the construction materials from Earth to the red planet with incredibly high cost, using Martian soil to construct a site on Mars is a superior choice.
Debate has tended to focus on chronology and iconography, with little consideration of the environmental relationships of rock carvings and how vegetation may help construct a site within its surrounding landscape.
I came out here to construct a site-specific installation in the woods that I would charge with creative energy, living in a tent while I built it, and I'm fucking serious about it.
Therefore, we constructed a sites-ligand interaction network using a bipartite graph to check the degree distributions of both binding sites and ligands.
Based on a QM6a Δ tku70 Δ pyr2 strain, Jørgensen et al. constructed a site-specific integration system in which the gene of interest is inserted at the ade2 locus together with pyr2 used as marker (26).
In constructing a sod house, a site had to be chosen that was close to good sources of sod.
A group called the Coalition of Concerned Cardozo Alumni constructed a Web site, shameoncardozo.com, and posted a letter declaring the group's outrage to the law school's board of overseers.
In Whittier, we constructed a central site at a subject home elevated on a hill.
