While various groups have attempted to construct synthetic pathways for 1-butanol production, efforts to construct a pathway that functions under aerobic conditions have met with limited success.
In this study, the apcB gene from a thermophilic cyanobacterium strain was cloned and ligated into an expression vector to construct a pathway for the biosynthesis of an allophycocyanin beta subunit (holo-apcBT) in Escherichia coli.
First, we chose an RBS optimization strategy, which is a promising metabolic engineering method that has been applied to construct a pathway for various compounds in previous reports (Sun et al. 2016; Veetil et al. 2017), to attenuate the expression of argF.
Eventually wishing to be one with the Gods, the children constructed a pathway to paradise.
He created the Beastmen, commanding them to forever fight the people of Vana'diel and occupy their minds, so these children would never have time to group together and construct a pathway like the ones before them.
This data was used to construct a pathway map, as mentioned above (Figure 2).
Li et al. [ 9] constructed a pathway cross-talk network (PCN) based on PPIs which connected between two pathways' components.
Biologists have to read a large number of published papers, interpret them and construct a pathway (Ananiadou et al., 2006).
Based on these studies, we identified proteins known to regulate or interact with RNASE5 and constructed a pathway gene set.
In an effort to describe and consolidate knowledge of pathways central to macrophage activation we have constructed a pathway diagram based on published literature.
