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This strategy was tested by constructing a library of polymers employing alkanoyloxy-substituted benzodithiophene (BDT) as the weak donor, and benzothiadiazole (BT) as the strong acceptor.
The RosettaAbInitio procedure begins by constructing a library of three- and nine-residue fragments from PDB structures with similar sequences, secondary structures, and profiles as stretches of the query sequence.
After constructing a library of LacI-GFP insertion proteins (LacI-GFPi) using TAGIT, we identified six sites in LacI that are tolerant to GFP insertion, including those previously identified by epitope insertion mutagenesis.
By constructing a library of cellular gates the authors have implemented simple logical functions such as OR, NOT and AND.
The randomized sensitivities are achieved by constructing a library of synthetic gene circuits with randomized control sequences (e.g., ribosome-binding sites) in the front element.
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This suggests it would be feasible to construct a library of DNA signatures linked with English family names.
Using 33 commercially available monomers it is theoretically possible to construct a library of over 40,000 distinct polymer compositions.
As a model we constructed a library of octapeptides and showed that the library is amenable to selection on cos-7 cells.
Utilizing this platform, we construct a library of orthogonal synthetic transcription factors (sTFs) and use these to wire synthetic transcriptional circuits in yeast.
Used to construct a library of promoters in E. coli Taylor et al. (2008) pUCG18P5bgaB Template for promoter library This study pMTL61110 E. coli/Geobacillus shuttle vector; AmpR, KanR.
The bio-scientists were aiming to construct a library of tens of thousands of small molecule candidates available in databases such as DrugBank, that have been screened against known targets using molecular modelling and tools (in this case, Autodock).
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