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We aim at constructing a curve network on the point cloud that will define the border of the various surface patches.
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Just as importantly, we see in Descartes' letter his attempt to expand the scope of legitimate geometrical constructions beyond straightedge and compass by appealing to the motions needed to construct a curve.
Standard primers were used to construct a curve of cDNA with known copy numbers, to which unknown samples were compared.
They did so by constructing a demand curve.
Quantification of DNA was achieved by constructing a standard curve of calculated Ct versus concentration for a set of DNA standards that were included in each run.
For the results scoring, a cut-off value of 0.7 IU/mL was chosen for all stimuli by constructing a ROC curve.
Quercetin was used as standard for constructing a calibration curve.
Primer-specific amplification efficiencies were determined by constructing a standard curve of serially diluted cDNA.
The efficiency of each primer set was first validated by constructing a standard curve through four serial dilutions.
Quantification was obtained by constructing a standard curve from serial dilutions of normal genomic DNA (ref 11 691 112 001; Roche, Meylan, France).
The efficiency of each primer pair was determined by constructing a standard curve from serial dilutions (Table 4).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com