Overall, strains were constructed which produce 146-fold (6595 µg/L) more vitamin than the wild-type and by far represents the highest yield reported.
An iron complex has been constructed which produces oxoferryl [Fe(IV =O] in combination with hydrogen peroxide [ 57], which is claimed to be potentially more specific than other ROS such as hydroxyl radical owing to lower diffusion.
The construct, which produces a GST fusion protein, was transformed into E. coli strain BL21Gold (DE3) pLysS (Novagen, Madison, WI).
Interestingly, in the case of constructs which produced a high level of cleavage, the fluorescent signals from both GFP and CherryFP were distributed throughout the cytoplasm, in the case of constructs which produced low levels of cleavage, the fluorescent signals were accumulated mostly around the nucleus.
Building on bio-logical devices that were modularised and rigorously characterised earlier, we co-transformed constructs which produce GFP (CARRY) from an HrpRS AND gate in a low copy plasmid, RFPasv (SUM) from hybrid promoters pBAD-Cl2B and pRHAB-Cl2B and λCl repressors from a pHrpL promoter in a high copy plasmid into E. coli (Fig. 6a).
In this study, an astaxanthin-biosynthesis Kluyveromyces marxianus strain Sm23 was first constructed, which could produce 31 µg/g DCW astaxanthin.
A piezo-electric droplet generator has been designed and constructed, which is capable of producing double, triple and hexagonally-arranged droplet trains.
The DNA fragment containing the eGFP coding sequence was directly inserted into ORF1 of pO-RFP to yield a dicistronic construct pGFP-RFP, which produced the target protein eGFP in ORF1 and the reporter protein mCherry in ORF2.
The DNA fragment containing extra strong RBS, ATG initiation codon and the mCherry coding sequence was directly ligated in pET-21a previously digested with HindIII and XhoI, forming a dicistronic construct pO-RFP, which produced mCherry in the second ORF (ORF2).
