The levels of Dm-AMP1 in transgenic lines were in the range of 0.74% to 0.92% in polyprotein construct whereas it was 0.43% to 0.57% in single-protein construct (Dm-AMP1) plants.
To investigate kinetics, a mechanistic pathway scheme with all established intermediates was constructed, whereas physical/chemical adsorption/desorption energies, geometries, barriers and rates for adsorbate elementary steps were calculated using plane-wave DFT.
Gender, being a man or a woman, is socially constructed, whereas sex, being male or female, is biologically defined.
Such criticism parallels the debate among gender theorists about whether we can distinguish sex from gender on the ground that gender is socially constructed whereas sex is not.
In other languages such as Korean, the situation is the opposite, and new pronouns can be constructed, whereas the number of adjectives is fixed.
Duplicate CCIH cohort TMA blocks that each contained single 0.6-mm cores sampled from representative paraffin blocks from each patient were constructed, whereas the RPCI TMA was constructed using duplicate cores from each patient in a single block.
2b-RAD libraries were prepared for two parents and 96 progenies by following the protocol developed by Wang et al. For the parents, standard BsaXI libraries were constructed, whereas for the progenies, reduced representation (RR) libraries were constructed using adaptors with 5′-NNT-3′ overhangs to target a subset of all BsaXI fragments in the C. farreri genome.
From this and other studies we can make the following conclusions and recommendations; in vivo (including intracellular) experiments and investigations into the protein kinase activity of PI 3-K should ideally be conducted with untagged constructs, whereas it is safe to carry out in vitro lipid kinase screening using tagged recombinant protein.
In nonmelanoma cell lines, the CMV promoter/enhancer (CMV p/e) was 969 times stronger than the Tyrex2 construct, whereas in melanoma cells it was only 2.6 times stronger.
Hybrids glauc1 and glauc2 showed a deletion of the T-DNA construct, whereas glauc3 and glauc7 retained it.
